TWiN 75: Arc, tau, and Alzheimer's

So was it, uh, hard to make a rat knockout? Often you don't hear about that.

Mostly mice, right?

Well, with CRISPR it's, it's pretty straightforward, but in this case-

Right

... we, you know, we outsourced it to a company, so they made it for us.

Okay.

Um, it's...

But yeah, it, it, it's kind of interesting.

The, whatever the, the, the embryonic, uh, stem cell technology that was developed for mouse transgenics-

Yeah

... is much harder to do in rats.

And I-

Right

...

I'm not, not exactly sure why.

Um, and you know-

So-

...

the technology now

... how do you, so how do you do the, the CRISPR? How do you do the germline CRISPR then?

So it's just that it's just recombination.

So they, they, you know, it's the same thing.

They, they take out, um...

They, they figure out the embryon- they take embryonic stem cells, inject the CRISPR guide RNA with the Cas9-

Hmm