Förster resonance energy transfer

It's more of this idea that it's aggregate-prone, and when it binds, uh, normal tau, it can induce aggregation.

Um, and so this, this was a, um, a FRET-based assay, so a fluorescent-based assay developed by Mark Diamond's group, where you have two forms of tau that have tags on them and-

Mm-hmm

...

They have a really cool way to address this.

So they have rat ventricular cardiomyocytes that produce a FRET-based cGMP biosensor.

So FRET is Forster resonance energy transfer.

It's a technique where two fluorescent molecules are attached to a protein that can sense cGMP, and when cGMP levels rise, the protein changes shape, it alters the distance between the fluorophores, and that changes the ratio of emitted light at two wavelengths, and you can watch cGMP levels rise and fall in real time in a living cell.