Could you tell us about the kind of imaging that you did, over what time scale you did this to pick up on atrophy?

So what we did is volumetric brain analysis.

We use a very established tool for that.

It's called FreeSurfer, and what you need is a T1-weighted image of the brain, typically a 3D image.

We did not scan the patients, but we asked our collaborators worldwide if they can send us their data, and these are all scans that were acquired in clinical routine.

This was not a prospective study, but we just collected the data that was available.

And as a next step, we then ran these volumetric analysis using FreeSurfer, and that had volumes for all relevant substructures.

Do we think that we are missing some IgLON5 cases?