Ron Brown
Former United States Secretary of Commerce
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Jul 6, 2026
Episode 68: Peak Fitting, Deconvolution & AI in Chromatography
29:40

M. Farooq WahabGUEST
And I've used the term digital chromatography for that [laughs] because you're doing post-processing of the data digitally on your computer.
R
29:49Ron BrownGUEST
If you look at, this is anything from a chromatography going across the x-axis, the second variable is now the wavelength.
R
29:56Ron BrownGUEST
So each one of these traces, as I, as I would go across in time, would be a separate chromatogram.
R
30:03Ron BrownGUEST
So this particular data set here is, obviously there's a large number of components here.
R
30:09Ron BrownGUEST
Some are present at a very trace level, some are present at a very predominant level.
R
30:14Ron BrownGUEST
But all of these are what can be done in a single chromatographic separation if you just save your 3D data.
R
30:23Ron BrownGUEST
So if you just save the 3D DAD PDA data in a liquid chromatography, you can use this particular information to try to separate the peaks that are completely coeluting And for example, if they completely coelute at 220 or 2- 210, uh, one of the wavelengths where just about everything absorbs, it's very possible there's two other wavelengths out there where one component has a principal absorbance, the other has nothing.
22 MINS LATER