Is there any standard pipelines people use or anything like that?

Yeah.

So about.

10 years ago, there's a few sort of competing pipelines, which came out doing very similar things.

So the sort of the early ish approaches to NGS sequenced ancient DNA is very much a reference based mapping.

So, you know, taking the reads, cleaning them up, removing adapters, aligning to reference genome.

variant calling and so on.

But firstly, let's ask you, James, like who are you writing it for? And what were you expecting the audience to be able to achieve by the end of it?

Dinosaurs,

yeah.

So I would classify... three major, three to four major areas of ancient DNA.

The biggest one by far is human population genomics.

Who had sex with who and when basically.

It's not my most interesting topic, but a lot of people are interested in like where they came from.

So that gets a lot of the money nowadays.

What's helped the most in all of that? And are there other like secondary things like getting into, or is some of your stuff getting into like academic textbooks? Like is the next generation learning this?